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2015
Zoology(Major)
Paper : 5.4
(Biological Techniques & Biostatistics)
Full Marks : 60
Time : 3 hours
1. Answer the following questions in brief : 1x6=6
(a) Which instrument measures colour of a sample?
(b) What is the principle of cryopreservation?
(c) What is radiotracer?
(d) What is the. measure of central tendency?
(e) What is the application of fluorescence microscope?
(f) Write the principle of geometric mean.
2. Answer any five of the following questions : 2x5=10
(a) What is the basic difference between colorimeter and spectrophotometer?
(b) Distinguish between paper chromatography and thin-layer chromatography.
(c) Distinguish between median and mode.
(d) Describe the basic. principle of ultracentrifugation.
(e) Describe the significance of regression.
(f) Write the application of ANOVA in biological science.
3. Answer any three of the following questions : 5x3=15
(a) Describe the uses of spectrophotometer in zoology.
(b) Define harmonic mean with suitable example.
(c) Describe ‘less than ogive’ with suitable example.
(d) The following are the 6 groups of planktons collected from three
sampling stations of river Brahmaputra. Represent the data using bar diagram
:
Planktons |
Site-1 |
Site-2 |
Site-3 |
Bacillariophyceae |
55 |
64 |
69 |
Chlorophyceae |
105 |
98 |
78 |
Cyanophyceae |
59 |
61 |
62 |
Copepoda |
53 |
52 |
61 |
Rotifers |
43 |
38 |
32 |
Cladocera |
23 |
25 |
27 |
4. Calculate median from the following. data : 4
x |
f |
0-10 |
6 |
10-20 |
14 |
20-30 |
16 |
30-40 |
27 |
40-50 |
22 |
50-60 |
15 |
60-70 |
18 |
Or
What do you mean by sampling units? Describe the merits and demerits of
random sampling techniques. 1+3=4
5. Describe the principle and application of thin-layer chromatography.
2+3=5
Or
Write the procedure and application of agarose gel electrophoresis. 5
6. What is independent sample t-test? Describe when to use the independent
sample t-test.Discuss independent. sample t-test with
Suitable example. 2+2+6=10
Or
Describe the principle and procedure of autoradiography for study or to
understand cellular function. Describe its various applications. 3+3+4=10
7. Describe the principle of electron microscopy. Write the procedure and
application of Transmission Electron Microscopy (TEM). 2+4+4=10
Or
Discuss the principle and procedure of cryopreservation techniques for
preservation of egg and sperm. 5+5=10