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2014
Zoology(Major)
Paper : 5.4
(Biological Techniques & Biostatistics)
Full Marks : 60
Time : 3 hours
1. Answer the following questions in brief: 1x6=6
(a) Which instrument measures concentration of hydrogen ions in a solution?
(b) What is ‘line of best fit ' ?
(c) Why is spectrophotometer also known as photometer?
(d) Define ‘less than ogive’.
(e) What is the modern application of ultracentrifugation?
(f) Write the disadvantages of harmonic mean.
2. Answer any five of the following questions : 2x5=10
(a) Describe the merits and demerits of geometric mean.
(b) Describe the basic. principle of SDS-PAGE gel electrophoresis.
(c) Describe the importance of statistics in Zoology.
(d) Describe the principle and procedures of dehydration step of
histological techniques.
(e) Describe the significance of ‘t-test’ and ‘ANOVA' .
(f) Distinguish between phase contrast and fluorescence microscopy.
3. Answer any three of the following questions : 5x3=15
(a) Describe the applications of radioisotopes in Zoology.
(b) Define standard deviation with suitable example.
(c) Describe the working principles of SEM and TEM. Comments on the
advantages and disadvantages of both.
(d) The following are the numbers of family, genus and species of 9 orders
of fishes collected from river Brahmaputra. Draw three pie diagram to
represent family, genus and species-wise percentage of the collected data :
Order |
Family |
Genus |
Species |
Cypriniformes |
3 |
17 |
25 |
Siluriformes |
7 |
10 |
14 |
Perciformes |
5 |
6 |
10 |
Clupeiformes |
1 |
1 |
1 |
Cyprinodontiformes |
1 |
2 |
3 |
Osteoglossiformes |
1 |
1 |
1 |
Mugiliformes |
1 |
2 |
2 |
Tetraodontiformes |
1 |
1 |
1 |
Beloniformes |
1 |
1 |
1 |
4. Calculate mode from the following data using grouping and analyzing table
: 4
x | f |
15 | 4 |
20 | 6 |
25 | 12 |
30 | 10 |
35 | 7 |
40 | 3 |
Or
Write the basic principle of fluorescence microscope. How is specimen
prepared for examination in the said microscope: 2+2=4
5. What is autoradiography? Describe the techniques of conventional
autoradiography. 2+3=5
Or
What is goodness of fit? Discuss chi-square with suitable example.
2+3=5
6. What do you mean by sample? Describe different types of random sampling
technique used in Biology. 3+7=10
Or
What do you mean by correlation? Describe different types of correlation.
Describe Karl Pearson’s coefficient of correlation with
suitable example. 2+3+5=10
7. What is basic principle of chromatography? Describe briefly different
types of chromatographic technique used in Biology.Write the application of
HPLC. 2+6+2=10
Or
What is the difference between high-speed centrifuge and ultracentrifuge?
Write the principle and procedure of ultracentrifuge.
3+7=10