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2016
Zoology(Major)
Paper : 5.4
(Biological Techniques & Biostatistics)
Full Marks : 60
Time : 3 hours
1. Answer the following questions in brief: 1x7=7
(a) State the significance of pH value.
(b) Write the Lambert’s law.
(c) What is limit of resolution?
(а) Express the relation between RCF and RPM in centrifugation.
(e) Define vitrification.
(f) What is more than ogive?
(g) What do you mean by goodness of fit?
2. Answer briefly the following : 2х4=8
(a) Distinguish between UV-Vis Spectrophotometry and atomic absorption
spectrophotometry.
(b) State the significance of chi-square test.
(c) Discuss the factors which affect the sectioning in microtomy.
(d) Distinguish between geometric mean and harmonic meari.
3. Answer any three from the following : 3x5=15
(a) Discuss the basic difference in working principles of scanning
electron microscope and transmission electron microscope. 5
(b) What is autoradiography? State the Principles and application of
“autoradiography. 1+2+2=5
(с) Discuss the importance of stati in biology. CP
(d) The following are the seven groups of zooplankton collected from two
sampling stations of river Brahmaputra :
Planktons |
Sampling station-I |
Sampling station-II |
Daphnia sp. |
28 |
32 |
Maina sp. |
41 |
56 |
Bosmina sp. |
34 |
43 |
Keratella sp. |
76 |
85 |
Brachionus sp. |
86 |
101 |
Cyclops sp. |
112 |
117 |
Nauplius sp. |
69 |
74 |
Represent the data of each site using pie-diagram. 2½x2½=5
(e) Calculate mode from the following data: 5
x | f |
0-10 | 15 |
10-20 | 17 |
20-30 | 32 |
30-40 | 13 |
40-50 | 12 |
50-60 | 10 |
4. What is regression equation? What is line of best fit? Describe
regression equation Yon X with suitable example. 2+2+6=10
Ог
What is correlation? State the different types of correlation. Describe
the Karl Pearson’s Coéfficient Of correlation with suitable example.
2+2+6=10
5. Describe the process of fixation, block preparation, sectioning and
staining in microtomy. 2+3+2+3=10
Or
Describe the principle and application of paper chromatography and column
chromatography. 5+5=10
6. Write the principles and application of pH meter and ultracentrifuge.
5+5=10
Or
Describe the working. principle and application of fluorescence and phase
contrast microscopy with suitable illustrations. 5+5=10