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2018
Zoology(Major)
Paper : 5.4
(Biological Techniques & Biostatistics)
Full Marks : 60
Time : 3 hours3
1. Answer the following questions very briefly :1x7=7
(a) What is molar extinction coefficient?
(b) Define mode.
(c) What is critical point drying?
(d) Write the definition of Pearson’s chi-square test.
(e) What is immunostain?
(f) Define correlation coefficient.
(g) What is native gel electrophoresis?
2. Answer any four of the following questions :2x4=8
(a) What is silver stain? Write’ the importance of silver stain in
histological study.
(b) Distinguish between arithmetic mean and median.
(c) What is pH electrode? Write the working principle of pH electrode.
(d) Describe about the machine language of a computer.
(e) What is vibratome? Write about the use of vibratome.
(f) What is linear regression in biostatistics? '
(g) Write the basic principle of centrifuge. Mention the use of
ultracentrifuge in biological research.
3. Answer any five of the following questions :3x5=15
(a) What is freezing microtome? Write the advantages and disadvantages of
freezing microtome,
(b) What are the computer-aided techniques used in biology? Write the
importance of computer-aided technique in medicine.
(c) What is ion-exchange chromatography? Write the basic principle of
ion-exchange chromatography.
(d) What is the meaning of standard error of mean? Write the. difference
between standard deviation and variance.
(e) Define autoradiography. Write the importance of receptor autoradiography
in biology.
(f) Establish the relation between wavelength of light and magnification.
(g) Write the characteristics of data set suitable for chi-square test. 4
4. What is fluorescence dye? How does a fluorescence dye work? Write the
importance of fluorescence dye in biological research.
3+4+3=10
Or
Describe biological database. Write the importance of biological database
for taxonomic study. 5+5=10
5. What is HPLC? Describe about the reverse phase HPLC. Mention about the uses of HPLC in. different fields. 3+3+4=10
Or
Describe different sampling techniques used in biological research. 10
6. (a) Define cryoprotectant. Write the necessity of sperm preservation.
2+3=5
(b) What is absorption spectroscopy? Discuss the disadvantages of UV-visible
spectroscopy. 2+3=5
Or
(c) What do you mean by graphical representation of data? Write the
significance of pie chart. 2+3=5
(d) What is cumulative frequency curve? Draw a cumulative frequency curve
from the data given below : 2+3=5
Mark Range |
Number Of Students |
0-10 |
5 |
10-20 |
9 |
|
16 |
30-40 |
22 |
40-50 |
26 |
50-60 |
18 |
60-70 |
11 |
70-80 |
6 |
80-90 |
4 |
90-100 |
3 |